Molecular Mass Determination

Mass spectrometry deals with the accurate mass measurement by producing charged molecular species in vacuum and their separation by magnetic and electric fields based on mass to charge (m/z) ratio. MALDI is an efficient process for generating gas-phase ion of peptides and proteins for mass spectrometric detection. TOF is a mass analyzer in which the flight time of the ion from the source to the detector is correlated to the m/z of the ion. MALDI mass spectrometry is an attractive approach for determination of accurate molecular weight of intact proteins.